Model wavefront sensor for adaptive confocal microscopy

Martin J. Booth; Mark A. A. Neil; Tony Wilson

doi:10.1117/12.384178

2 May 2000 Model wavefront sensor for adaptive confocal microscopy

Martin J. Booth, Mark A. A. Neil, Tony Wilson

Proceedings Volume 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII; (2000) https://doi.org/10.1117/12.384178
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

A confocal microscope permits 3D imaging of volume objects by the inclusion of a pinhole in the detector path which eliminates out of focus light. This configuration is however very sensitive to aberrations induced by the specimen or the optical system and would therefore benefit from an adaptive optics approach. We present a wavefront sensor capable of measuring directly the Zernike components of an aberrated wavefront and show that it is particularly applicable to the confocal microscope since only those wavefronts originating in the focal region contribute to the measured aberration.

Citation Download Citation

Martin J. Booth, Mark A. A. Neil, and Tony Wilson "Model wavefront sensor for adaptive confocal microscopy", Proc. SPIE 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII, (2 May 2000); https://doi.org/10.1117/12.384178

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