External optical imaging of freely moving mice with green fluorescent protein-expressing metastatic tumors

Meng Yang; Eugene Baranov; Hiroshi Shimada; A. R. Moossa; Robert M. Hoffman

doi:10.1117/12.384220

27 April 2000 External optical imaging of freely moving mice with green fluorescent protein-expressing metastatic tumors

Meng Yang, Eugene Baranov, Hiroshi Shimada, A. R. Moossa, Robert M. Hoffman

Author Affiliations +

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384220
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We report here a new approach to genetically engineering tumors to become fluorescence such that they can be imaged externally in freely-moving animals. We describe here external high-resolution real-time fluorescent optical imaging of metastatic tumors in live mice. Stable high-level green flourescent protein (GFP)-expressing human and rodent cell lines enable tumors and metastasis is formed from them to be externally imaged from freely-moving mice. Real-time tumor and metastatic growth were quantitated from whole-body real-time imaging in GFP-expressing melanoma and colon carcinoma models. This GFP optical imaging system is highly appropriate for high throughput in vivo drug screening.

Citation Download Citation

Meng Yang, Eugene Baranov, Hiroshi Shimada, A. R. Moossa, and Robert M. Hoffman "External optical imaging of freely moving mice with green fluorescent protein-expressing metastatic tumors", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384220

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