Two-photon fluorescence confocal microscopy with a passively Q-switched Nd:YAG microchip laser

Colin J. H. Brenan; Peter T. C. So

doi:10.1117/12.384222

27 April 2000 Two-photon fluorescence confocal microscopy with a passively Q-switched Nd:YAG microchip laser

Colin J. H. Brenan, Peter T. C. So

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384222
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Of increasing interest is miniature and portable instrumentation for non- or minimally-invasive functional microscopy. Two-photon fluorescence confocal microscopy is one optical image modality not yet exploited in this context primarily because of the large physical size of the laser light sources needed to stimulate the two-photon absorption process. As a possible alternative, we report on the successful application of light at 1064 nm from a passively, Q-switched Nd:YAG microchip laser for two-photon fluorescence microscopy of biological samples. The high peak power in a small optomechanical package makes the microchip laser an attractive starting point for development of portable two-photon fluorescence imaging system for medical endoscopy and other remote sensing applications.

Citation Download Citation

Colin J. H. Brenan and Peter T. C. So "Two-photon fluorescence confocal microscopy with a passively Q-switched Nd:YAG microchip laser", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384222

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