Efficient detection and identification of single biomolecules by surface-enhanced Raman scattering

Steven R. Emory; Richard A. Keller

doi:10.1117/12.383358

21 April 2000 Efficient detection and identification of single biomolecules by surface-enhanced Raman scattering

Steven R. Emory, Richard A. Keller

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383358
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Advances in ultra sensitive instrumentation have allowed the detection of single fluorescence molecules in solution, on dielectric surfaces, and in low-temperature solids. However, these studies are limited by the requirement of a high fluorescence quantum yield and do not often provide sufficient spectroscopic information for identification. These problems are addressed by surface-enhanced Raman scattering (SERS) which provides highly resolved vibrational information, does not suffer form rapid photo bleaching, and eliminates the need for fluorescent tagging of nonfluorescent species. Recent studies of single silver nanoparticles reveal intrinsic enhancement factors as large as 10¹⁵. These giant enhancement factors allow single- molecule SERS spectra to be obtained using visible or near- IR excitation. The detection efficiency of experiments using visible excitation has thus far ben relatively low. Approaches to detecting single labeled and unlabeled biomolecules and to increasing the overall detection efficiency of single-molecule SERS with visible excitation are presented.

Citation Download Citation

Steven R. Emory and Richard A. Keller "Efficient detection and identification of single biomolecules by surface-enhanced Raman scattering", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383358

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