High-throughput single-molecule DNA screening based on electrophoresis

Michael R. Shortreed; Hanlin Li; Edward S. Yeung

doi:10.1117/12.383331

21 April 2000 High-throughput single-molecule DNA screening based on electrophoresis

Michael R. Shortreed, Hanlin Li, Edward S. Yeung

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383331
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

In disease diagnosis, DNA and proteins are distinguished based on charge and hydrodynamic radius. Many protein assays and DNA assays relevant to cancer diagnosis are based on electrophoresis. However, standard protocols are not only slow but also insensitive. We successfully demonstrated a high-throughput imaging approach that allows determination of the individual electrophoretic mobilities of many molecules at a time. Each measurement on ly requires a few ms to compete. This opens up the possibility of screening single copies of DNA or proteins within single biological cells for disease markers without performing polymerase chain reaction or other biological amplification.

Citation Download Citation

Michael R. Shortreed, Hanlin Li, and Edward S. Yeung "High-throughput single-molecule DNA screening based on electrophoresis", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383331

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