Single molecule probes of lipid membrane dynamics

Chad E. Talley; Robert C. Dunn

doi:10.1117/12.383346

21 April 2000 Single molecule probes of lipid membrane dynamics

Chad E. Talley, Robert C. Dunn

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383346
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Single molecule fluorescence measurements are used to characterize the local dynamics in model lipid films of DPPC. Analysis of emission trajectory autocorrelations reveals a dependence in the single molecule emission fluctuations which is correlated with the surface pressure of the lipid monolayer. Comparison of DPPC monolayers transferred onto mica at (pi) equals 5 mN/m and (pi) equals 30 mN/m exhibit characteristic single molecule fluctuation times of 700 ms and 1.30 s, respectively. These fluctuation times are correlated with the order in the film. Comparison with recent near-field measurements of probe molecule orientation and AFM measurements of film topography, however, indicate that the lipid phase surrounding the probe molecule remains the same from (pi) equals 5 mN/m to 30 mN/m. The increase in the characteristic emission fluctuation times with increasing surface pressure, therefore, reflects a decrease in the freedom surrounding a single molecule. These fluctuations are consistent with long range concerted motions of the lipid tailgroups in the lipid film.

Citation Download Citation

Chad E. Talley and Robert C. Dunn "Single molecule probes of lipid membrane dynamics", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383346

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