Molecular beacon biosensors for DNA/RNA analysis

Xiaohong Fang; Sheldon Schuster; Xiaojing Liu; Tiffany Correll; Peng Zhang; Ruby Tapec; Swadeshmukul Santra; Monde Qhobosheanne; Jane Hua Lou; Weihong Tan

doi:10.1117/12.380499

22 March 2000 Molecular beacon biosensors for DNA/RNA analysis

Xiaohong Fang, Sheldon Schuster, Xiaojing Liu, Tiffany Correll, Peng Zhang, Ruby Tapec, Swadeshmukul Santra, Monde Qhobosheanne, Jane Hua Lou, Weihong Tan

Author Affiliations +

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380499
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We have developed a variety of novel DNA biosensors using a new class of oligonucleotide probe, molecular beacon (MB). MB has the fluorescence signal transduction mechanism built within the molecules. It can report the presence of specific nucleic acids with high sensitivity and excellent selectivity. Biotinylated MBs have been designed and synthesized for immobilization onto silica surface through avidin-biotin binding. The effect of the avidin-biotin bridge on the MB hybridization has been studied. Our result shows that using streptavidin has less effect than using avidin in MB hybridization. Two kinds of fiber optical DNA sensors have been prepared and characterized: a fiber optic evanescent wave sensor and a submicrometer optical fiber sensor. The sensors are rapid,stable, highly selective, reproducible and regenerable. They have been applied to detect specific DNA and mRNA sequences and to the study of the DNA hybridization kinetics. Silica nanoparticles have also been used for MB immobilization in order to prepare a large quantity of nanometer sized DNA/RNA biosensors.

Citation Download Citation

Xiaohong Fang, Sheldon Schuster, Xiaojing Liu, Tiffany Correll, Peng Zhang, Ruby Tapec, Swadeshmukul Santra, Monde Qhobosheanne, Jane Hua Lou, and Weihong Tan "Molecular beacon biosensors for DNA/RNA analysis", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380499

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