Low-coherence interference microscopy at high numerical apertures

Colin J. R. Sheppard; Maitreyee Roy; Peter Svahn

doi:10.1117/12.390567

4 July 2000 Low-coherence interference microscopy at high numerical apertures

Colin J. R. Sheppard, Maitreyee Roy, Peter Svahn

Proceedings Volume 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications; (2000) https://doi.org/10.1117/12.390567
Event: Photonics Taiwan, 2000, Taipei, Taiwan

Abstract

A white-light Linnik interference microscope using high numerical aperture optics has been constructed. The system uses a tungsten halogen source and Kohler illumination with separate control over field and aperture stops, so that experiments can be conducted with a range of different operating conditions. Infinity tube length objectives are used in the two arms. Images are recorded with a CCD camera. Different algorithms have been investigated for extraction of information from the image data. These are basd on phase stepping, which is achieved based on the principle of the geometric phase, using a polarizing beam splitter, a quarter wave plate and a rotating polarizer. Image information extracted from the visibility of the fringes and also from the phase of the interference fringes has been investigated.

Citation Download Citation

Colin J. R. Sheppard, Maitreyee Roy, and Peter Svahn "Low-coherence interference microscopy at high numerical apertures", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); https://doi.org/10.1117/12.390567

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available