Multiphoton microspectroscopy of biological specimens

Bai-Ling Lin; Fu-Jen Kao; Ping Chin Cheng; Chi-Kuang Sun; RangWu Chen; YiMin Wang; JianCheng Chen; Yung-Shun Wang; Tzu-Ming Liu; Mao-Kuo Huang

doi:10.1117/12.390530

4 July 2000 Multiphoton microspectroscopy of biological specimens

Bai-Ling Lin, Fu-Jen Kao, Ping Chin Cheng, Chi-Kuang Sun, RangWu Chen, YiMin Wang, JianCheng Chen, Yung-Shun Wang, Tzu-Ming Liu, Mao-Kuo Huang

Author Affiliations +

Proceedings Volume 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications; (2000) https://doi.org/10.1117/12.390530
Event: Photonics Taiwan, 2000, Taipei, Taiwan

Abstract

The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro- spectroscopy of biological specimen at high spatial resolution. Chloroplasts in mesophyll protoplast of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.

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Bai-Ling Lin, Fu-Jen Kao, Ping Chin Cheng, Chi-Kuang Sun, RangWu Chen, YiMin Wang, JianCheng Chen, Yung-Shun Wang, Tzu-Ming Liu, and Mao-Kuo Huang "Multiphoton microspectroscopy of biological specimens", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); https://doi.org/10.1117/12.390530

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