Characterization of biofilms with a fiber optic spectrometer

S. Krautwald; A. Tonyali; B. Fellerhoff; Hilmar Franke; A. Tamachkiarov; T. Griebe; H. C. Flemming

doi:10.1117/12.406344

15 December 2000 Characterization of biofilms with a fiber optic spectrometer

S. Krautwald, A. Tonyali, B. Fellerhoff, Hilmar Franke, A. Tamachkiarov, T. Griebe, H. C. Flemming

Proceedings Volume 4087, Applications of Photonic Technology 4; (2000) https://doi.org/10.1117/12.406344
Event: 2000 International Conference on Application of Photonic Technology (ICAPT 2000), 2000, Quebec City, Canada

Abstract

Optical sensing is one promising approach to monitor bioflims in an early stage. Generally, natural bioflims are quite inhomogeneous, therefore we start the investigation with suspensions of dead bacteria in water as a simple model for a bioflim. An experimental arrangement based on a white light fiber optic spectrometer is used for measuring the density of a thin film with a local resolution in the order of several tim. The method is applied with model biofilms. In a computer controlled procedure reflectance spectra may be recorded at different positions in the x-y plane. Scanning through thin suspension regions of bacteria between glass plates allows an estimation of the refractive index of bacteria. Taking advantage of the light collecting property of the glass substrate a simple measurement of the fluorescence with local resolution is demonstrated as well.

Citation Download Citation

S. Krautwald, A. Tonyali, B. Fellerhoff, Hilmar Franke, A. Tamachkiarov, T. Griebe, and H. C. Flemming "Characterization of biofilms with a fiber optic spectrometer", Proc. SPIE 4087, Applications of Photonic Technology 4, (15 December 2000); https://doi.org/10.1117/12.406344

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available