High-numerical-aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy

Yoshihiro Kawano; Chikara Abe; Teruo Kaneda; Yasushi Aono; Katsuyuki Abe; Keisuke Tamura; Susumu Terakawa

doi:10.1117/12.401623

29 September 2000 High-numerical-aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy

Yoshihiro Kawano, Chikara Abe, Teruo Kaneda, Yasushi Aono, Katsuyuki Abe, Keisuke Tamura, Susumu Terakawa

Author Affiliations +

Proceedings Volume 4098, Optical Devices and Diagnostics in Materials Science; (2000) https://doi.org/10.1117/12.401623
Event: International Symposium on Optical Science and Technology, 2000, San Diego, CA, United States

Abstract

We designed Apo 100X NA 1.65 and Plan Apo 60X NA 1.45 objective lenses. When we use three lenses for water- immersed specimens, the marginal angle available for the total internal reflection becomes larger compared with NA 1.40 objective lens. Therefore, the alignment of the laser beam to the objective lens becomes very easy. We also designed the TIRFM illuminator. A single mode fiber is connected between the laser and illuminator. The laser beam is conducted through the fiber and supplied to the illuminator with a pre-adjusted positioning. Almost no additional alignment of illumination light is necessary.

Citation Download Citation

Yoshihiro Kawano, Chikara Abe, Teruo Kaneda, Yasushi Aono, Katsuyuki Abe, Keisuke Tamura, and Susumu Terakawa "High-numerical-aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy", Proc. SPIE 4098, Optical Devices and Diagnostics in Materials Science, (29 September 2000); https://doi.org/10.1117/12.401623

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