Water structure and water/protein interactions in biological materials characterized by Raman spectroscopy

Ole Faurskov Nielsen; Carina Koch Johansson; Kirsten L. Jakobsen; Daniel H. Christensen; Mette R. Wiegell; Thorvald Pedersen; Monika Gniadecka; Hans Christian Wulf; Peter Westh

doi:10.1117/12.401624

29 September 2000 Water structure and water/protein interactions in biological materials characterized by Raman spectroscopy

Ole Faurskov Nielsen, Carina Koch Johansson, Kirsten L. Jakobsen, Daniel H. Christensen, Mette R. Wiegell, Thorvald Pedersen, Monika Gniadecka, Hans Christian Wulf, Peter Westh

Author Affiliations +

Proceedings Volume 4098, Optical Devices and Diagnostics in Materials Science; (2000) https://doi.org/10.1117/12.401624
Event: International Symposium on Optical Science and Technology, 2000, San Diego, CA, United States

Abstract

The R(v)-representation of the low-frequency Raman spectrum was used to investigate the low-frequency Raman spectrum of water. The advantages of using reduced representations in low-frequency Raman studies to display water structure are discussed. Tetrahedrically hydrogen bonded water molecules showed a characteristic low-frequency band with a peak maximum around 180 cm^-1. O-18 and O-17 isotopic substitution revealed that the corresponding vibrational mode mainly involves displacements of the oxygen atoms, but no significant hydrogen motion. This mode can be used to monitor the existence of water with a bulk-like structure in biological macromolecular materials. To test its applicability NIR-FT-Raman spectroscopy was used in studies of biopolymers in order to avoid fluorescence.

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Ole Faurskov Nielsen, Carina Koch Johansson, Kirsten L. Jakobsen, Daniel H. Christensen, Mette R. Wiegell, Thorvald Pedersen, Monika Gniadecka, Hans Christian Wulf, and Peter Westh "Water structure and water/protein interactions in biological materials characterized by Raman spectroscopy", Proc. SPIE 4098, Optical Devices and Diagnostics in Materials Science, (29 September 2000); https://doi.org/10.1117/12.401624

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