Interferometric investigations of the cosmonaut erythrocytes

Julia K. Novoderzhkina; Elena A. Streletskaya; Gennady I. Kozinets

doi:10.1117/12.405943

3 November 2000 Interferometric investigations of the cosmonaut erythrocytes

Julia K. Novoderzhkina, Elena A. Streletskaya, Gennady I. Kozinets

Proceedings Volume 4162, Controlling Tissue Optical Properties: Applications in Clinical Study; (2000) https://doi.org/10.1117/12.405943
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

Many biological objects, include blood cells, are optically transparent. They don't absorb light and only change optical path length by variations of object's thickness or its refractive index variations. The phase shifting interferometry is used for the visualization of the phase information and the obtaining quantitative properties of cells. Cells are fixed by glutaraldehyde and mounted in immersion liquid for reduction the influences of refraction. Blood cell's interferogram contains the information about space distribution refractive index. The phase-shift method was used for its reconstruction. This method includes record of four interferograms used different values of phase-shift of the reference wave. Many blood cells interferograms are obtained (lymphocytes and erythrocytes). The shape, size and density of cells are measurement. We suppose that these date will be ones of the early diagnostically criteria of hematological diseases. Experimental results for interferometric microscopy and tomography of blood cells are presented.

Citation Download Citation

Julia K. Novoderzhkina, Elena A. Streletskaya, and Gennady I. Kozinets "Interferometric investigations of the cosmonaut erythrocytes", Proc. SPIE 4162, Controlling Tissue Optical Properties: Applications in Clinical Study, (3 November 2000); https://doi.org/10.1117/12.405943

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