Hydration and stability of some globular proteins in the nonpolar medium in the presence of phosphatidilholine

Valeriy M. Klimovich; I. S. Gulay

doi:10.1117/12.411750

29 December 2000 Hydration and stability of some globular proteins in the nonpolar medium in the presence of phosphatidilholine

Valeriy M. Klimovich, I. S. Gulay

Author Affiliations +

Proceedings Volume 4203, Biological Quality and Precision Agriculture II; (2000) https://doi.org/10.1117/12.411750
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

Intention of present work is research the influence of non- polar medium and phosphatidilholine on stability of the macromolecules and hydration of cytohrom-C, tripsine and insulin by use of methods laser Raman and Infrared spectroscopy and isotope H/D exchange. It is shown, that the non-polar environment causes convertible changes of spatial pattern of macromolecules a protein degree of order of macromolecules as a result of which is increased. The presence of water at a system results in a converse effect. At interaction of phosphatidilcholin with the protondonors groups a protein will derivate complexes with a hydrogen bonds. Thereof quantity of aminoacidic oddments which are generatix a polar circuit of a plaited layer is augmented. The outcomes of the analysis of bands of compound tone of water testify to presence in a system of three varieties of water clusters distinguished by frequencies of libration oscillations. It is suspected, that the hydrophobic environment can cause reduction of movability of molecules of water in different clusters.

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Valeriy M. Klimovich and I. S. Gulay "Hydration and stability of some globular proteins in the nonpolar medium in the presence of phosphatidilholine", Proc. SPIE 4203, Biological Quality and Precision Agriculture II, (29 December 2000); https://doi.org/10.1117/12.411750

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