Light-emission-based biosensor for detection of food pathogens: a review

Ashutosh Singh; Rakesh K. Singh; Arun K. Bhunia

doi:10.1117/12.418723

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13 March 2001 Light-emission-based biosensor for detection of food pathogens: a review

Ashutosh Singh, Rakesh K. Singh, Arun K. Bhunia

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Proceedings Volume 4206, Photonic Detection and Intervention Technologies for Safe Food; (2001) https://doi.org/10.1117/12.418723
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

Rapid detection, identification and enumeration of pathogenic microorganisms is highly important to the food industry to address the food safety concerns. Biosensors are devices that promise to achieve these objectives, these analytical devices can accurately and selectively estimate the levels of foodborne pathogens. The most widely applied biosensors are based on the optical properties like absorption, fluorescence, reflection, refraction, dispersion etc. This review is done to provide an overview of optic based biosensor and their application in the area of food safety. Working principles of Surface plasmon resonance , resonant mirror and fiber optic based biosensor are described in the article. Resonant mirror based biosensor has been used for the estimation of S. aureus, it was proven selective for the strain being tested (Cowan-1). Using colloidal-gold conjugates in sandwich assay format increased the sensitivity of the assay. An integrated fiber optic based biosensor is used for detection of Salmonella typhimurium reducing the detection time to 30 min. Fiber optic based biosensors offer advantage of compactness, flexibility, resistance to electrical noise and small probe size.

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Ashutosh Singh, Rakesh K. Singh, and Arun K. Bhunia "Light-emission-based biosensor for detection of food pathogens: a review", Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); https://doi.org/10.1117/12.418723

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