Autofocus methods for automated microscopy

Xiaoqiang Wu; XinMing Liu; Yiming Zhou; Deyuan Tao; Xiaohai He; Qizhi Teng

doi:10.1117/12.403954

11 October 2000 Autofocus methods for automated microscopy

Xiaoqiang Wu, XinMing Liu, Yiming Zhou, Deyuan Tao, Xiaohai He, Qizhi Teng

Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403954
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China

Abstract

This paper introduced a basis principle of an autofocus system, including the hardware composition and the software flow chart. The most important work of an autofocus system is to construct or select a suitable focus function. We first discussed some traditional focus functions, pointed out their advantages and shortages. Then we proposed a new autofocus algorithm, which based on wavelet transform. We use the diagonal edge information of the result of the wavelet transform to form focus function. After many experiments we draw a conclusion that the wavelet transform method is superiority than others, use it can get a highly accuracy along with a fast speed, special in the biomedical microscopic image analysis. At the last of this paper we discussed the further work related to the improvement of hardware condition and the optimization of focus function.

Citation Download Citation

Xiaoqiang Wu, XinMing Liu, Yiming Zhou, Deyuan Tao, Xiaohai He, and Qizhi Teng "Autofocus methods for automated microscopy", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); https://doi.org/10.1117/12.403954

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