Experimental research of photodynamic diagnosis mediated by chemiluminescence reagents

Juan Wang; Da Xing; Yonghong Tang; Yonghong He

doi:10.1117/12.403979

11 October 2000 Experimental research of photodynamic diagnosis mediated by chemiluminescence reagents

Juan Wang, Da Xing, Yonghong Tang, Yonghong He

Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403979
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China

Abstract

There are some serious limitations existing in conventional photodynamic diagnosis (PDD), such as autofluorescence disturbance, weak red-band absorption efficacy of traditional photosensitizers and quite short penetration depth of excitation light. In this paper a novel method of PDD mediated by chemiluminescence reagents is presented in fabricated mixed samples. Photodynamic therapy (PDT) is believed to act via singlet oxygen (¹O₂) generated by energy from photosensitizers. 5-Fluoresceinyl Cypridina Luciferin Analog (FCLA), a chemiluminescence reagent, can react with singlet oxygen and emit chemiluminescence with peak wavelength about at 526 nm. In the experiments, FCLA was chosen as an optical reporter of localization of singlet oxygen produced from photosensitized reaction of Hematoporphyrin Derivative (HpD) excited by 630 nm laser. Experimental results, the measurement of fluorescence spectra and the detection of image from chemiluminescence, show that the mixed HpD and FCLA in biologic tissue mixture excited with the laser of 630 nm can emit detectable light signals.

Citation Download Citation

Juan Wang, Da Xing, Yonghong Tang, and Yonghong He "Experimental research of photodynamic diagnosis mediated by chemiluminescence reagents", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); https://doi.org/10.1117/12.403979

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available