Free-calcium distribution and calcium pulses in rat peripheral macrophages

Yanhua Yu; Da Xing; Yonghong Tang; Ying Jin

doi:10.1117/12.403973

11 October 2000 Free-calcium distribution and calcium pulses in rat peripheral macrophages

Yanhua Yu, Da Xing, Yonghong Tang, Ying Jin

Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403973
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China

Abstract

With Laser Confocal Scanning Microscope (LCSM) system, three aspects of characteristics of free cytoplasmic calcium in rat peripheral macrophages are studied. One is the Ca²⁺ concentration in different area in the same cell. Second is the Ca²⁺ concentration in the same area in different dividing stage. Third is the feature of calcium pulses evoked by Kcl or pH changing. The results show that even in one cell, the evolution of the Ca²⁺ concentration is not the same in a different area. In the same area, the nucleolus Ca²⁺ concentration in division breaking stage is much higher than that in division stage. From the experiment phenomena, we conclude that Kcl itself can not evoke calcium pulses in the unexcitable macrophage, but the change of pH can trig calcium pulses in the same cells.

Citation Download Citation

Yanhua Yu, Da Xing, Yonghong Tang, and Ying Jin "Free-calcium distribution and calcium pulses in rat peripheral macrophages", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); https://doi.org/10.1117/12.403973

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KEYWORDS

Calcium

Luminescence

Confocal microscopy

Microscopes

Liquids

3D image processing

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