Two-photon fluorescence imaging of rat aorta

Xingsun Lin; Fei Sun; Hui Ma; Jingbo Zhao; Lei Jin; Die Yan Chen

doi:10.1117/12.403959

11 October 2000 Two-photon fluorescence imaging of rat aorta

Xingsun Lin, Fei Sun, Hui Ma, Jingbo Zhao, Lei Jin, Die Yan Chen

Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403959
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China

Abstract

Two-photon excitation fluorescence microscopy has become increasingly popular in 3D fluorescence imaging of tissue samples. Observation depth is an important indicator for the performance of the technique. Using the principle of tissue optics, we analyzed quantitatively the effects of optical properties, such as absorption coefficient, scattering coefficient, and the scattering anisotropy factor of the tissue, to the observation depth. An analytical relation between the fluorescence and the observation depth has been derived and tested against experimental results for rat aorta elastic lamina.

Citation Download Citation

Xingsun Lin, Fei Sun, Hui Ma, Jingbo Zhao, Lei Jin, and Die Yan Chen "Two-photon fluorescence imaging of rat aorta", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); https://doi.org/10.1117/12.403959

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available