Excimer laser ablation of plasma polymers for cell and tissue culture applications

Helmut Thissen; Jason P. Hayes; Patrick G. Hartley; Graham Johnson; Erol C. Harvey; Hans J. Griesser

doi:10.1117/12.424405

6 April 2001 Excimer laser ablation of plasma polymers for cell and tissue culture applications

Helmut Thissen, Jason P. Hayes, Patrick G. Hartley, Graham Johnson, Erol C. Harvey, Hans J. Griesser

Proceedings Volume 4234, Smart Materials; (2001) https://doi.org/10.1117/12.424405
Event: Smart Materials and MEMS, 2000, Melbourne, Australia

Abstract

The two-dimensional control of cell adhesion is desired for a number of cell- and tissue culture applications. Thus, a suitable method for the two-dimensional control over surface chemistry, which leads to the display of cell-adhesive and non-adhesive signals is required. In our study, allylamine plasma polymer (ALAPP) deposition has been used to provide a cell-adhesive substrate, while additional grafting of poly(ethylene oxide) (PEO) on ALAPP surfaces has been used to prevent cell adhesion. Two-dimensional control over the surface chemistry was achieved using excimer laser ablation. Ablation experiments were carried out using a 248 nm excimer laser with energy densities of 17 - 1181 mJ/cm² and 1 - 16 pulses per area. Results obtained by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) show controlled thickness ablation of the plasma polymer and the additional PEO graft polymer. Cell culture experiments using bovine corneal epithelial cells show that two-dimensional control of cell adhesion can be achieved by using appropriate masks in the laser beam.

Citation Download Citation

Helmut Thissen, Jason P. Hayes, Patrick G. Hartley, Graham Johnson, Erol C. Harvey, and Hans J. Griesser "Excimer laser ablation of plasma polymers for cell and tissue culture applications", Proc. SPIE 4234, Smart Materials, (6 April 2001); https://doi.org/10.1117/12.424405

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available