Characterization of an in-vitro laser thrombolysis model

Abram D. Janis; Abby N. Nyara; Kenton W. Gregory M.D.; Lisa A. Buckley

doi:10.1117/12.427833

21 May 2001 Characterization of an in-vitro laser thrombolysis model

Abram D. Janis, Abby N. Nyara, Kenton W. Gregory M.D., Lisa A. Buckley

Author Affiliations +

Proceedings Volume 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI; (2001) https://doi.org/10.1117/12.427833
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Laser thrombolysis (LT) is under investigation as a safe and rapid therapy for arterial recanalization in acute embolic stroke. Clot formation is a complex process affected by many factors that lead to differences in strength and hemoglobin concentration between samples formed from whole blood. The strength of thrombus formed in vivo also varies with age. We have developed a reproducible and clinically relevant reconstituted clot model for the in vitro assessment of a laser thrombolysis system. Laser thrombolysis experiments were performed using a 577 nm 1 microsecond(s) ec pulsed dye laser at an energy of approximately 25 mJ and a repetition rate of 4 Hz. Laser ablation, tensile strength, scanning electron microscopic, and histological comparisons are made with other in vitro clot models. We have found that the tensile strength of this clot model is directly proportional to the fibrinogen concentration. Laser ablation and tensile pull test studies demonstrate that LT ablation efficiency (in (mu) g/mJ/pulse) is not significantly affected by differences in clot tensile strength. This agrees with previous studies using dye and gelatin. Our results provide support for the effective use of this laser thrombolysis system for the removal of clots of varied age and strength.

Citation Download Citation

Abram D. Janis, Abby N. Nyara, Kenton W. Gregory M.D., and Lisa A. Buckley "Characterization of an in-vitro laser thrombolysis model", Proc. SPIE 4244, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XI, (21 May 2001); https://doi.org/10.1117/12.427833

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