Scavengers modifying the phototoxicity induced by ALA-mediated photodynamic therapy

Adriana Casas; Christian Perotti; Haydee Fukuda; Alcira Batlle

doi:10.1117/12.424451

9 April 2001 Scavengers modifying the phototoxicity induced by ALA-mediated photodynamic therapy

Adriana Casas, Christian Perotti, Haydee Fukuda, Alcira Batlle

Proceedings Volume 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X; (2001) https://doi.org/10.1117/12.424451
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The exogenously stimulated formation of intracellularly generated Protoporphyrin IX, a precursor of heme, is becoming one of the fastest developing areas in the field of Photodynamic Therapy (PDT). We have examined the degree of protection of several scavengers, aminoacids and compounds related to glutathione metabolism, to the photodamage induced by 5-aminolevulinic acid (ALA)-mediated PDT, employing the LM2 cell line, derived from a mammary murine adenocarcinoma. We have exposed the cells to different concentrations of the scavengers, 24 before PDT, during PDT, and 19 hr after treatment. We defined the protection grade (PG) as the ratio between cell survival after ALA-PDT treatment in the presence of the protector and cell survival after ALA-PDT treatment. We found that L-tryptophan (PG=8.3 at 2mM), N-acetyl-L-cysteine (PG= 7.9 at 30 mM), L-cysteine (PG=7.81 at 8mM), S-adenosyl-L-methionine (PG= 7.86 at 8mM), melatonin (PG=6.81 at 8mM) and glycine (PG=6.8 at 40 mM) are the best protectors to PDT damage, followed by L-methionine (PG=4.38 at 0.8 mM), mannitol (PG=2.32 at 2 mM) and reduced glutathione (PG=3.41 at 0.8 mM), whereas oxidized glutathione does not exert any protection. The implications of these results in the photodamage induced by ALA-PDT is discussed.

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Adriana Casas, Christian Perotti, Haydee Fukuda, and Alcira Batlle "Scavengers modifying the phototoxicity induced by ALA-mediated photodynamic therapy", Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); https://doi.org/10.1117/12.424451

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KEYWORDS

Photodynamic therapy

Control systems

Oxygen

Hydrogen

Cancer

Cell death

In vitro testing

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