High-resolution optical tomographic imaging for soft biological tissues: an experimental study of wavelength dependence

Ruikang K. Wang; James B. Elder; Victoria Smith

doi:10.1117/12.427897

23 May 2001 High-resolution optical tomographic imaging for soft biological tissues: an experimental study of wavelength dependence

Ruikang K. Wang, James B. Elder, Victoria Smith

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Proceedings Volume 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V; (2001) https://doi.org/10.1117/12.427897
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

We report experimentally how the wavelength dependence affects the OCT imaging contrast and depth by the use of normal human and animal tissues in vitro. Two systems, using the light sources with central wavelengths at 820nm and 1310nm, respectively, were set-up and used in present work to study the light penetrating and imaging contrast dependence on wavelength. The tissue specimens used were colonic and stomach mucosa. The results confirmed that the longer wavelength suffers less scattering and absorption, thus penetrating deeper into the tissue, but pays a penalty of reduced imaging contrast. In general, both imaging depth as well as tissue contrast is determined by tissue absorption, scattering properties and the refractive indices in microscopic and macroscopic scale, which vary with wavelength. Thus, the choice of wavelengths used in the OCT system should be careful in terms of optimizing imaging depth, imaging contrast as well as differentiation between different tissue morphologies.

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Ruikang K. Wang, James B. Elder, and Victoria Smith "High-resolution optical tomographic imaging for soft biological tissues: an experimental study of wavelength dependence", Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); https://doi.org/10.1117/12.427897

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KEYWORDS

Tissues

Optical coherence tomography

Imaging systems

Refractive index

Scattering

Light scattering

Stomach

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