Ultrahigh-velocity resolution imaging of the microcirculation in-vivo using color Doppler optical coherence tomography

Siavash Yazdanfar; Andrew M. Rollins; Joseph A. Izatt

doi:10.1117/12.427887

23 May 2001 Ultrahigh-velocity resolution imaging of the microcirculation in-vivo using color Doppler optical coherence tomography

Siavash Yazdanfar, Andrew M. Rollins, Joseph A. Izatt

Author Affiliations +

Proceedings Volume 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V; (2001) https://doi.org/10.1117/12.427887
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Color Doppler optical coherence tomography (CDOCT) is a method for noninvasive cross-sectional imaging of blood flow in vivo. In previous implementations, velocity estimates were obtained by measuring the frequency shift of discrete depth-resolved backscatter spectra, resulting in a velocity resolution on the order of 1 mm/s. We present a novel processing method that detects Doppler shifts calculated across sequential axial scans, enabling ultrahigh velocity resolution (~1 micron/s) flow measurement in scattering media. This method of sequential scan processing was calibrated with a moving mirror mounted on a precision motorized translator. Latex microspheres suspended in deuterium oxide were used as a highly scattering test phantom. Laminar flow profiles down to ~15 micron/s centerline velocity (0.02 cc/hr) were observed with a sensitivity of 1.2 micron/s. Finally, vessels on the order of 10 microns in diameter were imaged in living human skin, with a relative frequency sensitivity less than 4 x 10^-5. To our knowledge, these results are the lowest velocities ever measured with CDOCT.

Citation Download Citation

Siavash Yazdanfar, Andrew M. Rollins, and Joseph A. Izatt "Ultrahigh-velocity resolution imaging of the microcirculation in-vivo using color Doppler optical coherence tomography", Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); https://doi.org/10.1117/12.427887

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