Immunoaffinity-based biosensor for polycyclic aromatic hydrocarbons

C. Brent Bargeron; Richard C. Benson; Terry E. Phillips; Peter F. Scholl; Sala Abubaker; John D. Groopman; Paul T. Strickland

doi:10.1117/12.426743

22 May 2001 Immunoaffinity-based biosensor for polycyclic aromatic hydrocarbons

C. Brent Bargeron, Richard C. Benson, Terry E. Phillips, Peter F. Scholl, Sala Abubaker, John D. Groopman, Paul T. Strickland

Author Affiliations +

Proceedings Volume 4255, Clinical Diagnostic Systems; (2001) https://doi.org/10.1117/12.426743
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

An automated biosensor for 1-hydroxypyrene-glucuronide (OHPG) has been developed using a sensor platform initially designed for aflatoxin. The platform is based on the properties of immunoaffinity for sample purification and concentration, and fluorescence for detection. Experiments have demonstrated capture, wash, elution, and quantitation with very good results. The device is handheld and battery- driven, and can be miniaturized further. The bed volume of the column was 0.1 ml with a capacity of about 400 ng of the OHPG. Analysis time was 10 - 11 minutes. The sensitivity was about 0.5 ppb. The antibodies for the system were developed previously and recognize OHPG. The biosensor relies upon microprocessor-controlled minifluidics and fluorometry.

Citation Download Citation

C. Brent Bargeron, Richard C. Benson, Terry E. Phillips, Peter F. Scholl, Sala Abubaker, John D. Groopman, and Paul T. Strickland "Immunoaffinity-based biosensor for polycyclic aromatic hydrocarbons", Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); https://doi.org/10.1117/12.426743

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