Multiexcitation fluorescence and reflectance spectroscopy as a real-time guide to biopsy in vivo

Markus G. Mueller; Irene Georgakoudi; Adam Wax; Ramachandra R. Dasari; Christian Jost; Michael B. Wallace; Michael S. Feld

doi:10.1117/12.424525

16 April 2001 Multiexcitation fluorescence and reflectance spectroscopy as a real-time guide to biopsy in vivo

Markus G. Mueller, Irene Georgakoudi, Adam Wax, Ramachandra R. Dasari, Christian Jost, Michael B. Wallace, Michael S. Feld

Author Affiliations +

Proceedings Volume 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII; (2001) https://doi.org/10.1117/12.424525
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

A compact excitation-emission spectrofluorimeter system used to acquire tissue fluorescence and reflectance with fiber optic light delivery and collection is presented. Ten dye cells are pumped by a XeC1 excimer laser, creating laser excitation from 308 nm to 505nm, which is used to excite tissue fluorescence. A xenon-flash lamp is employed to obtain the reflectance spectrum of the corresponding tissue spot. Eleven fluorescence spectra and one white light diffuse reflectance spectrum are collected in vivo in 0.2 seconds and can be potentially analyzed in real time to obtain diagnostic information. The instrument can be used for early cancer detection. The measured fluorescence and reflectance spectra are used to model the intrinsic fluorescence of the tissue, which gives important insight into its biochemical composition (tryptophan, collagen, elastin, NADH, etc.) and metabolic state. Diffuse reflectance provides information about tissue morphology, in terms of the scatterhig and absorption coefficient. By subtracting the diffusely reflected component from the measured reflectance, light scattering spectroscopic (LSS) information due to single backscattering from epithelial cell nuclei can be obtained. LSS provides information about the size distribution of cell nuclei. The parameters give diagnostic information, which can be used to guide the physician in real time while taking biopsies of invisible tissue abnormalities.

Citation Download Citation

Markus G. Mueller, Irene Georgakoudi, Adam Wax, Ramachandra R. Dasari, Christian Jost, Michael B. Wallace, and Michael S. Feld "Multiexcitation fluorescence and reflectance spectroscopy as a real-time guide to biopsy in vivo", Proc. SPIE 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII, (16 April 2001); https://doi.org/10.1117/12.424525

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