Three-dimensional imaging with a quadrature tomographic microscope

Johnathan J. Stott; R. Eric Bennett; Carol M. Warner; Charles A. DiMarzio

doi:10.1117/12.424533

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16 April 2001 Three-dimensional imaging with a quadrature tomographic microscope

Johnathan J. Stott, R. Eric Bennett, Carol M. Warner, Charles A. DiMarzio

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Proceedings Volume 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII; (2001) https://doi.org/10.1117/12.424533
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

he quadrature tomographic microscope combines conventional microscopy with interferometric quadrature detection techniques to yield a simultaneous measurement of both the amplitude and phase of the electric field. This is of tremendous utility in imaging nearly-transparent object's since changes in phase can be directly attributed to changes in the objects index of refraction while the small amplitude changes provide additional structural information. In this paper we will discuss the progress we have made towards reconstructing three dimensional images from the measured two-dimensional complex electric field. By applying an artificial curvature to the measured field and back-propagating the Gaussian beams, we are able to digitally manipulate the imaging kernel and image different focal planes.

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Johnathan J. Stott, R. Eric Bennett, Carol M. Warner, and Charles A. DiMarzio "Three-dimensional imaging with a quadrature tomographic microscope", Proc. SPIE 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII, (16 April 2001); https://doi.org/10.1117/12.424533

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