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16 April 2001Three-dimensional imaging with a quadrature tomographic microscope
he quadrature tomographic microscope combines conventional microscopy
with interferometric quadrature detection techniques to yield a
simultaneous measurement of both the amplitude and phase of the electric
field. This is of tremendous utility in imaging nearly-transparent
object's since changes in phase can be directly attributed to changes in
the objects index of refraction while the small amplitude changes provide
additional structural information. In this paper we will discuss the
progress we have made towards reconstructing three dimensional images
from the measured two-dimensional complex electric field. By applying
an artificial curvature to the measured field and back-propagating the
Gaussian beams, we are able to digitally manipulate the imaging kernel
and image different focal planes.
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Johnathan J. Stott, R. Eric Bennett, Carol M. Warner, Charles A. DiMarzio, "Three-dimensional imaging with a quadrature tomographic microscope," Proc. SPIE 4261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VIII, (16 April 2001); https://doi.org/10.1117/12.424533