Two-photon investigation of calcium dynamics in dendritic spines during motility

Ania Majewska; Ayumu Tashiro; Rafael Yuste M.D.

doi:10.1117/12.424576

24 April 2001 Two-photon investigation of calcium dynamics in dendritic spines during motility

Ania Majewska, Ayumu Tashiro, Rafael Yuste M.D.

Proceedings Volume 4262, Multiphoton Microscopy in the Biomedical Sciences; (2001) https://doi.org/10.1117/12.424576
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Dendritic spines receive most excitatory inputs in the CNS and compartmentalize calcium. Spines also undergo rapid morphological changes, although the function of this motility is still unclear. We have investigated the effect of spine movement on spine calcium dynamics with two-photon photobleaching of enhanced Green Fluorescent Protein (EGFP) and calcium imaging of action potential-elicited transients in spines from layer 2/3 pyramidal neurons in mouse visual cortex slices. The elongation or retraction of the spine neck during spine motility alters the diffusional coupling between spine and dendrite and significantly changes calcium decay kinetics in spines. Our results demonstrate that the spine's ability to compartmentalize calcium is constantly changing.

Citation Download Citation

Ania Majewska, Ayumu Tashiro, and Rafael Yuste M.D. "Two-photon investigation of calcium dynamics in dendritic spines during motility", Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); https://doi.org/10.1117/12.424576

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