Adaptive functional support for bioreceptors based on protein layers

K. V. Kostyukevych; Evgenie F. Venger; E. V. Rengevich; Sergey A. Kostyukevych; S. V. Verevka; P. M. Boltovets; N. S. Dyachenko

doi:10.1117/12.429723

12 June 2001 Adaptive functional support for bioreceptors based on protein layers

K. V. Kostyukevych, Evgenie F. Venger, E. V. Rengevich, Sergey A. Kostyukevych, S. V. Verevka, P. M. Boltovets, N. S. Dyachenko

Author Affiliations +

Proceedings Volume 4425, Selected Papers from the International Conference on Optoelectronic Information Technologies; (2001) https://doi.org/10.1117/12.429723
Event: International Conference on Optoelectronic Information Technologies, 2000, Vinnytsia, Ukraine

Abstract

The formation of Au_xS_y interfacial layer by reactive annealing of gold films in H₂S atmosphere is investigated. This seems to be a technologically favorable technique for the large-scale and low-cost fabrication of nondestructive immobilization support for biological molecules. Formation of phases with different chemical functionality and surface topography as a function of reaction time was studied using Atomic Force Microscopy (AFM), Surface Plasmon Resonance (SPR) measurements and biomolecular interaction analysis [trypsin -- Soybean Trypsin Inhibitor (STI) reaction]. The results obtained confirm the classical two-step model for the sulfide phase formation during reactive annealing. This includes an intermediate formation of a dispersed phase of sulfur followed by its reconstruction to a close-packed sulfide layer. Adsorption of proteins onto a certain sulfide layer passes with retention of a native state of adsorbed molecules. The proposed strategy for formation of biochemical structures (gold/sulfide/proteins) on the surface of physical transducers open a new way for design and development of novel artificial smart sensing systems. They not only maintain optimal functioning of bioreceptors but also are responsive to their environment.

Citation Download Citation

K. V. Kostyukevych, Evgenie F. Venger, E. V. Rengevich, Sergey A. Kostyukevych, S. V. Verevka, P. M. Boltovets, and N. S. Dyachenko "Adaptive functional support for bioreceptors based on protein layers", Proc. SPIE 4425, Selected Papers from the International Conference on Optoelectronic Information Technologies, (12 June 2001); https://doi.org/10.1117/12.429723

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available