Antidiabetics and diuretics show phototoxicity in HaCaT cells

Edgar Selvaag M.D.; Anita B. Petersen; Robert Gniadecki; Tine Thorn; Hans Christian Wulf

doi:10.1117/12.446520

26 October 2001 Antidiabetics and diuretics show phototoxicity in HaCaT cells

Edgar Selvaag M.D., Anita B. Petersen, Robert Gniadecki, Tine Thorn, Hans Christian Wulf

Proceedings Volume 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy; (2001) https://doi.org/10.1117/12.446520
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

The antidiabetics tolbutamide, glibenclamide, and glipizide, and the diuretics bendroflumethiazide, butizide, furosemide, hydrochlorothiazide, and trichlormethiazide were investigated for potential phototoxicity in the HaCaT cell line. The cells were incubated with the drugs and then exposed to UVA1 irradiation. The effects of the antioxidants L-ascorbic acid, and (alpha) -tocopherol on oxidative DNA damage were assessed. Bendroflumethiazide, furosemide, hydrochlorothiazide, trichlormethiazide, or tolbutamide induced dose-dependent phototoxicity. Cells incubated with bendroflumethiazide, tolbutamide, and glibenclamide, and irradiated with UVA1 demonstrated an increased oxidative DNA damage. Pre-treatment with L-ascorbic acid, or (alpha) -tocopherol, suppressed the UVA-induced DNA damage in cells incubated with 1 mM of bendroflumethiazide, furosemide, glibenclamide, glipizide, tolbutamide, and trichloromethiazide, further implying the involvement of reactive oxygen species in the phototoxic DNA damage. These results may indicate a link between phototoxic and photocancerogenic potential of the sulfonamide-derived oral antidiabetic and diuretic drugs, as it has previously been recognized for psoralen, chlorpromazine, and fluoroquinolones. Excessive exposure to UV light may be deleterious for patients treated with these drugs.

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Edgar Selvaag M.D., Anita B. Petersen, Robert Gniadecki, Tine Thorn, and Hans Christian Wulf "Antidiabetics and diuretics show phototoxicity in HaCaT cells", Proc. SPIE 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy, (26 October 2001); https://doi.org/10.1117/12.446520

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KEYWORDS

Ultraviolet radiation

Bioalcohols

Oxygen

Photodynamic therapy

Skin cancer

Auroras

Biomedical optics

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