Paper
23 January 2002 Chemically enhanced D96N-mutant bacteriorhodopsin film as an advanced optical material
Bing Liang, Baofang Li, Long Jiang
Author Affiliations +
Abstract
The combination of the genetic engineering and chemical additive has been used in an attempt to obtain much longer M-state lifetime of bacteriorhodopsin-PVA film. Different compositions of D96N-mutant bacteriorhodopsin-PVA film with the diaza-15-crown-5 (1,4,10-trioxa-7,13- diazacyclopentadecane) additive were prepared and their spectral and kinetic transformation were investigated by absorbance spectroscopy. As the molecular ratio of BRD96N/diaza-15-crown-5 ranging from 1:50 - 1:150, the decay of the M-state was slowed down gradually. The fitting of the M-state decay kinetics curves needs a three-exponential- function to get sufficiently small residuals. At the highest additive concentration, the photochromism time of BRD96N film could last as long as one and a half hours. This provides a benefit to the BRD96N as a candidate material for optical applications under ambient conditions.
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Bing Liang, Baofang Li, and Long Jiang "Chemically enhanced D96N-mutant bacteriorhodopsin film as an advanced optical material", Proc. SPIE 4459, Photorefractive Fiber and Crystal Devices: Materials, Optical Properties, and Applications VII, and Optical Data Storage, (23 January 2002); https://doi.org/10.1117/12.454032
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KEYWORDS
Absorption

Absorbance

Genetic engineering

Molecules

Chromophores

Lithium

Optical storage

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