High-resolution element mapping inside biological samples using fluorescence microtomography

Christian G. Schroer; Boris Benner; Til Florian Guenzler; Marion Kuhlmann; Bruno Lengeler; Walter H. Schroeder; Arnd J. Kuhn; Alexandre S. Simionovici; Anatoly A. Snigirev; Irina Snigireva

doi:10.1117/12.452850

7 January 2002 High-resolution element mapping inside biological samples using fluorescence microtomography

Christian G. Schroer, Boris Benner, Til Florian Guenzler, Marion Kuhlmann, Bruno Lengeler, Walter H. Schroeder, Arnd J. Kuhn, Alexandre S. Simionovici, Anatoly A. Snigirev, Irina Snigireva

Author Affiliations +

Proceedings Volume 4503, Developments in X-Ray Tomography III; (2002) https://doi.org/10.1117/12.452850
Event: International Symposium on Optical Science and Technology, 2001, San Diego, CA, United States

Abstract

Sample preparation for element analysis of many biological tissues is difficult to achieve and prone to introduce contamination. Using x-ray fluorescence element microtomography (XFEMT) the element distribution on a virtual section across the sample can be determined with a resolution in the micrometer range. Fluorescence microtomograms of two plant samples are shown, demonstrating the possibility to map physiologically relevant ions, trace elements, and heavy metal pollutants at the cellular level. Attenuation effects inside the plant are corrected by a self-consistent tomographic reconstruction technique.

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Christian G. Schroer, Boris Benner, Til Florian Guenzler, Marion Kuhlmann, Bruno Lengeler, Walter H. Schroeder, Arnd J. Kuhn, Alexandre S. Simionovici, Anatoly A. Snigirev, and Irina Snigireva "High-resolution element mapping inside biological samples using fluorescence microtomography", Proc. SPIE 4503, Developments in X-Ray Tomography III, (7 January 2002); https://doi.org/10.1117/12.452850

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