Electrochemiluminescence immunoassay of P53 from human SWO-38 glioma cell

Guihong Yan; Yonghong He; Da Xing; Yadong Wei; Shici Tan

doi:10.1117/12.462550

12 April 2002 Electrochemiluminescence immunoassay of P53 from human SWO-38 glioma cell

Guihong Yan, Yonghong He, Da Xing, Yadong Wei, Shici Tan

Proceedings Volume 4536, International Workshop on Photonics and Imaging in Biology and Medicine; (2002) https://doi.org/10.1117/12.462550
Event: International Workshop on Photonics and Imaging in Biology and Medicine, 2001, Wuhan, China

Abstract

Electrochemiluminescence (ECL) technique for bimolecular detection offers ultra-high sensitivity and good signal-to- noise ratio. In this work, an ECL reaction and detection system were established. We used a big working electrode and a single photon counter to heighten the detection limit. The results showed that the detection limit of the system is 1/1000 fmol/L free labeled Ru(bpy)₃ ²⁺. The concentration dependence of ECL intensity was not linear in the range of low concentration (fmol/L level) free labeled Ru(bpy)₃ ²⁺. While in pmol/L level, the ECL intensity was linear. Ru(bpy)₃ ²⁺ + TPA (tripropylmine) ECL reaction system was used to indicate P53 protein from human SWO-38 cell line, which was established from grade 1-11 glioma tumor. This method could have potential applications in early-stage tumor diagnosis and environmental inspection.

Citation Download Citation

Guihong Yan, Yonghong He, Da Xing, Yadong Wei, and Shici Tan "Electrochemiluminescence immunoassay of P53 from human SWO-38 glioma cell", Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); https://doi.org/10.1117/12.462550

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