Multichannel optical biosensors for label-free high-throughput screening

Petr I. Nikitin; Boris Goshkov; Mikhail V. Valeiko; Sergei Nikitin

doi:10.1117/12.456063

14 February 2002 Multichannel optical biosensors for label-free high-throughput screening

Petr I. Nikitin, Boris Goshkov, Mikhail V. Valeiko, Sergei Nikitin

Proceedings Volume 4578, Fiber Optic Sensor Technology and Applications 2001; (2002) https://doi.org/10.1117/12.456063
Event: Environmental and Industrial Sensing, 2001, Boston, MA, United States

Abstract

An optical method has been proposed and successfully tested for direct detection of biochemical reactions on a surface, which is insensitive to variations of the radiation intensity and refractive index of a solution. The method is based on detection of the spectrum of the reflected or transmitted radiation modulated by the interference in a sensitive layer of large thickness (several tens and hundreds of microns), which can be a microscope cover glass with a deposited receptor layer. A change in the phase of the interference pattern in this spectrum is used as an information signal about a change in the thickness of the sensitive layer caused by a biochemical reaction. Single- and multichannel (up to 96 channels) devices have been designed to study reactions of binding and detachment of proteins in real time. The root-mean-square noise of the prototypes expressed in the layer thickness was 3 pm.

Citation Download Citation

Petr I. Nikitin, Boris Goshkov, Mikhail V. Valeiko, and Sergei Nikitin "Multichannel optical biosensors for label-free high-throughput screening", Proc. SPIE 4578, Fiber Optic Sensor Technology and Applications 2001, (14 February 2002); https://doi.org/10.1117/12.456063

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