Chemiluminescence detection from sonodynamic action in vitro and in vivo

Yonghong He; Da Xing; Guihong Yan; Ken-ichi Ueda

doi:10.1117/12.446658

25 October 2001 Chemiluminescence detection from sonodynamic action in vitro and in vivo

Yonghong He, Da Xing, Guihong Yan, Ken-ichi Ueda

Proceedings Volume 4597, Biophotonics Instrumentation and Analysis; (2001) https://doi.org/10.1117/12.446658
Event: International Symposium on Photonics and Applications, 2001, Singapore, Singapore

Abstract

In this work, chemiluminescence method was engaged for the first time to detect the active oxygen spices during sonocynamic action both in vitro and in vivo. We used CLA derivatives, which can efficiently react with singlet oxygen (¹O₂) or superoxide anion (O₂^-) to emit light, and luminol, which can be oxidized by a variety of free radicals to emit photons, to real-timely detect oxygen free radical formation in the sonosensitization of two sonosensitizer ATX-70 and HpD. The results show that ¹O₂ is involved in the sonosensitization. The mechanism of sonosensitizing was discussed. In vivo experiments, tumor imaging by sonodynamic chemiluminescence detection methods was established. This method could have potential applications in clinics for early-stage tumor diagnosis.

Citation Download Citation

Yonghong He, Da Xing, Guihong Yan, and Ken-ichi Ueda "Chemiluminescence detection from sonodynamic action in vitro and in vivo", Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); https://doi.org/10.1117/12.446658

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