Evaluation of variations of biomolecular constituents in human skin in vivo by near-infrared Raman spectroscopy

Zhiwei Huang; Haishan Zeng; Calum E. MacAulay; Iltefat Hamzavi; David I. McLean M.D.; Harvey Lui M.D.

doi:10.1117/12.446645

25 October 2001 Evaluation of variations of biomolecular constituents in human skin in vivo by near-infrared Raman spectroscopy

Zhiwei Huang, Haishan Zeng, Calum E. MacAulay, Iltefat Hamzavi, David I. McLean M.D., Harvey Lui M.D.

Author Affiliations +

Proceedings Volume 4597, Biophotonics Instrumentation and Analysis; (2001) https://doi.org/10.1117/12.446645
Event: International Symposium on Photonics and Applications, 2001, Singapore, Singapore

Abstract

A portable and rapid near-infrared (NIR) Raman spectroscopy system together with a tissue Raman probe was developed and utilized to acquire in vivo skin Raman spectra at 785 nm excitation. We demonstrated that good quality in vivo skin Raman spectra free of interferences of fiber fluorescence and silica Raman signals can be acquired within 5 seconds. Distinct Raman peaks in the 800-1800 cm^-1 range can be discerned clearly from various skin sites of the body. The intensity ratio of the Raman band at 1655 cm^-1 to that at 1445 cm^-1 was used to evaluate the variation of the protein/lipid depositions in the skin. The results show that the regional variations in the molecular composition and structure can be determined in vivo, suggesting that NIR Raman spectroscopy will be useful to non-invasively measure important biochemical parameters of human skin.

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Zhiwei Huang, Haishan Zeng, Calum E. MacAulay, Iltefat Hamzavi, David I. McLean M.D., and Harvey Lui M.D. "Evaluation of variations of biomolecular constituents in human skin in vivo by near-infrared Raman spectroscopy", Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); https://doi.org/10.1117/12.446645

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