Does light scattering affect the OCT quantitation of redox state of cytochrome oxidase in bone tissue?

Xiangqun Xu; Ruikang K. Wang; Alicia El Haj

doi:10.1117/12.470486

14 June 2002 Does light scattering affect the OCT quantitation of redox state of cytochrome oxidase in bone tissue?

Xiangqun Xu, Ruikang K. Wang, Alicia El Haj

Proceedings Volume 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI; (2002) https://doi.org/10.1117/12.470486
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

In our previous report, we have presented the possibility of optical coherence tomography (OCT) to monitor the redox state of mitochondria enzyme Cytochrome oxidase (CytOx) in bone tissue. The previous results showed that reduction of the enzyme in periosteal tissue leads to a change in attenuation coefficient of 1.68 +/- 0.67mm^-1 by OCT measurements. The new results from cultured cells fixed in 300 (mu) l agarose plug showed the difference in attenuation coefficient is 0.26+-0.10 mm^-1 (n = 9) for 7x10⁶ astrocytoma cells and 0.28+-0.13 mm^-1 (n = 7) for 20x10⁶ astrocytoma cells in agarose plug, respectively between cells with oxidised and reduced enzyme at 820nm. A decrease in attenuation coefficient of 0.35+-0.09 mm^-1 (n = 4) for 10 million SKMES cells in agarose was also observed with the redox shift of CytOx. The absorption coefficient of the oxidized-reduced form of CytOx is measured approximately 8.4+-1.5x10^-3/mm (n=3) and 8.2+-1.0x10^-3/mm (n=3) at 820nm for astrocytoma cells and rat periosteum respectively by means of a biochemical assay. Thereby it can be seen that the change in attenuation coefficient of cultured cells with redox shift of CytOx mainly results from the scattering change.

Citation Download Citation

Xiangqun Xu, Ruikang K. Wang, and Alicia El Haj "Does light scattering affect the OCT quantitation of redox state of cytochrome oxidase in bone tissue?", Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); https://doi.org/10.1117/12.470486

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