Two-photon 3D mapping of tisssue endogenous fluorescence species based on fluorescence emission spectra

Lily Laiho Hsu; Thomas M. Hancewicz; Peter D. Kaplan; Peter T. C. So

doi:10.1117/12.470702

17 June 2002 Two-photon 3D mapping of tisssue endogenous fluorescence species based on fluorescence emission spectra

Lily Laiho Hsu, Thomas M. Hancewicz, Peter D. Kaplan, Peter T. C. So

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470702
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of tissue structure and biochemistry. While autofluorescence is observed in many tissue types, the identities and distributions of these fluorophores have not been completely characterized. Image guided spectral analysis is being developed to aid in extracting spectroscopic components from two-photon images. This methodology is being applied to the study of human skin. In ex vivo specimens, the overall bulk emission spectrum of the skin, the layer-resolved emission spectra of the stratum corneum, stratum spinosum, basal layer, and dermis, and the emission spectra of surgically exposed dermis have been measured. From the image guided spectral analysis, it was determined that there are approximately five factors that contribute to most of the luminescence signals from human skin. The autofluorescent species identified include tryptophan, NAD(P)H, melanin (or localizing species), and elastin. The collagen matrix contributes to a second harmonic signal.

Citation Download Citation

Lily Laiho Hsu, Thomas M. Hancewicz, Peter D. Kaplan, and Peter T. C. So "Two-photon 3D mapping of tisssue endogenous fluorescence species based on fluorescence emission spectra", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470702

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