Modeling ECM fiber formation: structure information extracted by analysis of 2D and 3D image sets

Jun Wu; Sherry L. Voytik-Harbin; David L. Filmer; Christoph M. Hoffman; Bo Yuan; Ching-Shoei Chiang; Jennis Sturgis; Joseph Paul Robinson

doi:10.1117/12.467831

15 May 2002 Modeling ECM fiber formation: structure information extracted by analysis of 2D and 3D image sets

Jun Wu, Sherry L. Voytik-Harbin, David L. Filmer, Christoph M. Hoffman, Bo Yuan, Ching-Shoei Chiang, Jennis Sturgis, Joseph Paul Robinson

Author Affiliations +

Proceedings Volume 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX; (2002) https://doi.org/10.1117/12.467831
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Recent evidence supports the notion that biological functions of extracellular matrix (ECM) are highly correlated to its structure. Understanding this fibrous structure is very crucial in tissue engineering to develop the next generation of biomaterials for restoration of tissues and organs. In this paper, we integrate confocal microscopy imaging and image-processing techniques to analyze the structural properties of ECM. We describe a 2D fiber middle-line tracing algorithm and apply it via Euclidean distance maps (EDM) to extract accurate fibrous structure information, such as fiber diameter, length, orientation, and density, from single slices. Based on a 2D tracing algorithm, we extend our analysis to 3D tracing via Euclidean distance maps to extract 3D fibrous structure information. We use computer simulation to construct the 3D fibrous structure which is subsequently used to test our tracing algorithms. After further image processing, these models are then applied to a variety of ECM constructions from which results of 2D and 3D traces are statistically analyzed.

Citation Download Citation

Jun Wu, Sherry L. Voytik-Harbin, David L. Filmer, Christoph M. Hoffman, Bo Yuan, Ching-Shoei Chiang, Jennis Sturgis, and Joseph Paul Robinson "Modeling ECM fiber formation: structure information extracted by analysis of 2D and 3D image sets", Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); https://doi.org/10.1117/12.467831

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available