Delayed luminescence technique: a probe for vital processes in biological systems

Monica Ballerini; Marziale Milani; Lorenzo Ferraro; Matteo Zabeo; Francesco Musumeci

doi:10.1117/12.468351

28 May 2002 Delayed luminescence technique: a probe for vital processes in biological systems

Monica Ballerini, Marziale Milani, Lorenzo Ferraro, Matteo Zabeo, Francesco Musumeci

Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468351
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Delayed Luminescence is a well established technique based on the illumination of biological sample and on the subsequent count of the number of photons re-emitted by the sample after the light source has been switched off together with their spectral distribution. Investigations have been performed on yeast cells and algae so that correlations can be established between biological activities and physical parameters of the samples. Moreover nonlinear mechanisms of interaction between optical fields and cells can be figured out. Further investigations will be reported on yeast cell samples deposited on paper filters after irradiation by soft X-rays. The results will be discussed by cross correlating the experimental evidence from Delayed Luminescence with those obtained by metabolic activity recording. Luminescence and Delayed Luminescence are strictly correlated with early insurgence of morphological alterations of normal or pathological nature in cells and tissues; a novel technique for morphological analysis has been developed by means of Focused Ion Beam machines. A straightforward approach to morphology at the nanoscale both of membranes and cellular inner structures is then made possible. The final aim is an experimental set up for an early and reliable detection technique for neoplastic cells and tissues sorting.

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Monica Ballerini, Marziale Milani, Lorenzo Ferraro, Matteo Zabeo, and Francesco Musumeci "Delayed luminescence technique: a probe for vital processes in biological systems", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468351

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KEYWORDS

Luminescence

Yeast

Photons

Tissues

X-rays

Electromagnetism

Ion beams

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