Flow cytometric monitoring of hormone receptor expression in human solid tumors

Awtar Krishan

doi:10.1117/12.468346

28 May 2002 Flow cytometric monitoring of hormone receptor expression in human solid tumors

Awtar Krishan

Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468346
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Hormone receptor expression in human breast and prostate tumors is of diagnostic and therapeutic importance. With the availability of anti-estrogen, androgen and progesterone antibodies, immunohistochemistry has become a standard tool for determination of receptor expression in human tumor biopsies. However, this method is dependent on examination of a small number of cells under a microscope and the data obtained in most cases is not quantitative. As most of the commercially used anti-hormone antibodies have nuclear specificity, we have developed methods for isolation and antigen unmasking of nuclei from formalin fixed/paraffin embedded archival human tumors. After immunostaining with the antibodies and propidium iodide (for DNA content and cell cycle analysis), nuclei are analyzed by multiparametric laser flow cytometry for hormone receptor expression, DNA content, aneuploidy and cell cycle determination. These multiparametric methods are especially important for retrospective studies seeking to correlate hormone receptor expression with clinical response to anti-hormonal therapy of human breast and prostate tumors.

Citation Download Citation

Awtar Krishan "Flow cytometric monitoring of hormone receptor expression in human solid tumors", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468346

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KEYWORDS

Tumors

Receptors

Prostate

Breast

Autoregressive models

Flow cytometry

Luminescence

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