Theoretical investigation of the signal-to-noise ratio for different fluorescence lifetime imaging techniques

Kjell Carlsson; Johan Philip

doi:10.1117/12.468362

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28 May 2002 Theoretical investigation of the signal-to-noise ratio for different fluorescence lifetime imaging techniques

Kjell Carlsson, Johan Philip

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Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468362
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Using Monte-Carlo methods, we have investigated the signal- to-noise ratio obtainable for different fluorescence lifetime imaging methods. Quantum noise limited performance and mono-exponential decays were assumed. We have also investigated the importance of parameter choice and implementation for the different methods. In addition, our simulations were in many cases compared with analytical theoretical investigations. The results from the simulations proved to be in good agreement with the theoretical results. It was found that all the investigated lifetime imaging methods have the potential to produce a high signal-to-noise ratio, but careful attention must be paid to implementation method and parameter choice in order to get optimal results.

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Kjell Carlsson and Johan Philip "Theoretical investigation of the signal-to-noise ratio for different fluorescence lifetime imaging techniques", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468362

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