Contaminant effects on cellular metabolic differential pressure curve: a quantitative analysis

Marziale Milani; Monica Ballerini; Lorenzo Ferraro; Matteo Zabeo; Massimo Barberis; Maria Cannone; Venera Faraone

doi:10.1117/12.469774

4 June 2002 Contaminant effects on cellular metabolic differential pressure curve: a quantitative analysis

Marziale Milani, Monica Ballerini, Lorenzo Ferraro, Matteo Zabeo, Massimo Barberis, Maria Cannone, Venera Faraone

Proceedings Volume 4625, Clinical Diagnostic Systems: Technologies and Instrumentation; (2002) https://doi.org/10.1117/12.469774
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The possibility of using a pressure monitoring system based on differential pressure sensors to detect contaminant effects on cellular cultures metabolic activity is discussed using Saccharomyces cerevisiae cell cultures: differential pressure curves' shape, starting slope and maximum are affected both by physical and chemical contamination. Aim of the present study is the investigation of the effects generated by a 72h exposition of Saccharomyces cerevisiae, human lymphocytes and AHH1 cellular line cultures to 50Hz, 60(mu) T electromagnetic field. No significant differences have been recorded between irradiated and control yeast samples. On other hand irradiated lymphocytes samples, cultures in a PHA medium, grow less than control ones, but exhibit a greater metabolic activity: changes in the exposure system configuration influence neither sample growth differences nor metabolic response variations between control and irradiated samples. Control and irradiated lymphocyte samples, without PHA in culture medium, show the same behavior both during irradiation and metabolic test. AHH1 control and irradiated samples show no difference both in growth percentage during irradiation and in metabolic test. Different cell cultures respond to the same stimulus in different manners.

Citation Download Citation

Marziale Milani, Monica Ballerini, Lorenzo Ferraro, Matteo Zabeo, Massimo Barberis, Maria Cannone, and Venera Faraone "Contaminant effects on cellular metabolic differential pressure curve: a quantitative analysis", Proc. SPIE 4625, Clinical Diagnostic Systems: Technologies and Instrumentation, (4 June 2002); https://doi.org/10.1117/12.469774

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