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21 June 2002 Biomedical applications of radiative decay engineering
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Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002)
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States
Fluorescence spectroscopy is a widely used research tool in biochemistry and has also become the dominant method enabling the revolution in medical diagnostics, DNA sequencing and genomics. In this forward-looking article we describe a new opportunity in fluorescence, radiative decay engineering (RDE). By RDE we mean modifying the emission of fluorophores or chromophores by a nearby metallic surface, the most important effect being an increase in the radiative decay rate. We describe the usual effects expected form increase in the radiative rates with reference to the biomedical applications of immunoassay and DNA hybridization. We also present experiments which show that metallic particles can increase the quantum yield of low quantum yield fluorophores, increase fluorophore photostability and increase the distance for resonance energy transfer. And finally we show that proximity to silver particles can increase the intensity of the intrinsic fluorescence from DNA.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Joseph R. Lakowicz, Ignacy Gryczynski, Joanna Malicka, Yibing Shen, and Zygmunt Gryczynski "Biomedical applications of radiative decay engineering", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002);

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