Miniaturization of ultra-high-throughput screening assays into 1536-well format

James R. Beasley; Paul M. McCoy; Tiffany Walker; David A. Dunn

doi:10.1117/12.472060

21 June 2002 Miniaturization of ultra-high-throughput screening assays into 1536-well format

James R. Beasley, Paul M. McCoy, Tiffany Walker, David A. Dunn

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472060
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Assay miniaturization and the implementation of high-density 1536 micro-well screening increases the speed and efficiency of screening and lead discovery. To serve this need, a platform of miniaturizable assay technologies has been assembled for specific biological targets. This platform will enable initiation and completion of uHTS screens in a straightforward and expeditious manner. For kinases, we have examined assays using several technologies including DELFIA, HTR-FRET, FP, EFC, and FMAT. This presentation compares these technologies for the measurement of typical tyrosine kinase activity in 1536-well format. Quality parameters such as assay reproducibility, signal: background ratio, Z factor, and assay sensitivity were calculated and compared. Additionally, the relative merits of each of these technologies were assessed in terms of assay miniaturization, ease of development, ultimate screening capability, efficiency, and cost.

Citation Download Citation

James R. Beasley, Paul M. McCoy, Tiffany Walker, and David A. Dunn "Miniaturization of ultra-high-throughput screening assays into 1536-well format", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472060

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