Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays

Phan T. Tran; Ellen R. Goldman; George P. Anderson; J. Matthew Mauro; Hedi M. Mattoussi

doi:10.1117/12.459733

18 March 2002 Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays

Phan T. Tran, Ellen R. Goldman, George P. Anderson, J. Matthew Mauro, Hedi M. Mattoussi

Author Affiliations +

Proceedings Volume 4636, Nanoscience Using Laser-Solid Interactions; (2002) https://doi.org/10.1117/12.459733
Event: High-Power Lasers and Applications, 2002, San Jose, California, United States

Abstract

Colloidal CdSe-ZnS semiconductor quantum dots (QDs) have tunable narrow-band luminescence emission and high resistance to photodegradation, properties that make them promising for use as fluorescent labels in biotechnological applications such as fluoro-immunoassays and in cellular imaging. We have developed efficient methods for conjugating these inorganic fluorophores to antibodies via the use of a molecular adaptor protein, the B2 domain of streptococcal protein G appended with an electrostatic interaction domain, which acts as a bridge between the QD fluorophore and antibodies. In this approach, coupling of the adaptor to the QD is driven by electrostatic self-assembly, while bridging to the antibody is driven by specific interactions. In the present study, we present fluoro-immunoassay studies employing QD-antibody conjugates for the detection of low levels of the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). We will also explore the use of self-assembled QD-protein-receptor complexes in Foerster energy transfer assays, where the QD emission can be altered by a quenching dye receptor in a QD-protein-receptor conjugate.

Citation Download Citation

Phan T. Tran, Ellen R. Goldman, George P. Anderson, J. Matthew Mauro, and Hedi M. Mattoussi "Application of luminescent CdSe-ZnS quantum dot bioconjugates in immuno- and fluorescence-quenching assays", Proc. SPIE 4636, Nanoscience Using Laser-Solid Interactions, (18 March 2002); https://doi.org/10.1117/12.459733

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Proteins

Luminescence

Nanocrystals

Quantum dots

Energy transfer

Absorption

Receptors

Show All Keywords

Keywords/Phrases

Search In:

Publication Years