Biospecific assembling of gold nanoparticles with protein or oligonucleotide linkers as studied by light scattering and extinction spectra

Vladimir A. Bogatyrev; Lev A. Dykman; Yaroslav M. Krasnov; Vladimir K. Plotnikov; Nikolai G. Khlebtsov

doi:10.1117/12.475632

16 July 2002 Biospecific assembling of gold nanoparticles with protein or oligonucleotide linkers as studied by light scattering and extinction spectra

Vladimir A. Bogatyrev, Lev A. Dykman, Yaroslav M. Krasnov, Vladimir K. Plotnikov, Nikolai G. Khlebtsov

Author Affiliations +

Proceedings Volume 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III; (2002) https://doi.org/10.1117/12.475632
Event: Saratov Fall Meeting 2001, 2001, Saratov, Russian Federation

Abstract

In this work, we report on the optical properties of colloidal gold bioconjugates obtained by attaching protein molecules or oligonucleotide probes to the surface of the particles. Addition of complementary components to the bioconjugate probe results in a specific assembling of conjugates and can be monitored by light scattering and extinction spectra. To this end, we have developed a special attachment to the Specord M-40 spectrophotometer and a corresponding measurement procedure called by us differential light scattering spectroscopy. This method is suitable for the nearly simultaneous recording of extinction and static light scattering spectra of a simple that possesses overlapping spectral bands of absorption and light scattering.

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Vladimir A. Bogatyrev, Lev A. Dykman, Yaroslav M. Krasnov, Vladimir K. Plotnikov, and Nikolai G. Khlebtsov "Biospecific assembling of gold nanoparticles with protein or oligonucleotide linkers as studied by light scattering and extinction spectra", Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, (16 July 2002); https://doi.org/10.1117/12.475632

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