Fourier-transform phase-modulation fluorometer

Tetsuo Iwata; Takeshi Kamada; Tsutomu Araki

doi:10.1117/12.530349

19 November 2003 Fourier-transform phase-modulation fluorometer

Tetsuo Iwata, Takeshi Kamada, Tsutomu Araki

Proceedings Volume 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life; (2003) https://doi.org/10.1117/12.530349
Event: 19th Congress of the International Commission for Optics: Optics for the Quality of Life, 2002, Florence, Italy

Abstract

We propose a novel phase-modulation fluorometer by which fluorescence decay waveform can be obtained. In the fluorometer, the modulation frequency of the excitation light source is varied from f_min to f_max continuously within a time interval of T. The resultant sinusoidal fluorescence waveform is Fourier-transformed to obtain the amplitude and phase spectra. The ratio of the amplitude spectrum and the difference of the phase spectrum over those of the reference spectra that are obtained from a non-fluorescent material are calculated, respectively, and the data are inverse-Fourier-transformed to obtain the fluorescence decay waveform. We carried out numerical simulations and fundamental experiments using an electric RC differential circuit and a white light-emitting diode (LED). We call the method Fourier-transform phase-modulation fluorometry.

Citation Download Citation

Tetsuo Iwata, Takeshi Kamada, and Tsutomu Araki "Fourier-transform phase-modulation fluorometer", Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); https://doi.org/10.1117/12.530349

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