CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 4916 > Article
Paper
12 September 2002 Computer analysis method for determining protein relative molecular weight in gel electrophoresis images
Sixiang Zhang, Weiling Liu, Duogang Ran, Jiancai Wang, Xiaoxiao Li
Author Affiliations +
Proceedings Volume 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment; (2002) https://doi.org/10.1117/12.482926
Event: Photonics Asia, 2002, Shanghai, China
Abstract
The technology of computer analysis for gel electrophoresis images is based on the computer image analysis and gel electrophoresis. Particle in complex mixture have different molecular weight and with different charge. Migration distances of sample zones are compared with the position of standards and the molecular size of unknowns is determined. By this way, biology particle and molecule with different compound can be separated from other. Digital camera can acquire image and process digital transact, then based on the digital image to realize automatic identification, sign, molecular weight calculate. For the convenient of observe and acquire, sample pre band on ethidium bromide stained agarose gels. The instrument is mainly made up of UV light source, digital camera and camera obscura etc. Formulas for calculate of protein's molecular weight and mobility in polyacrylamide gel is connected with standard curve and the relative mobility of band. After many experiments the conclusion can be deduced that the change of electrophoresis start point and front edge (m, n, r's value) will result in the change of K and b. Unknown samples' molecular weight only have relation with the standard sample's molecular weight and the value of r1, r2 and r3.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Sixiang Zhang, Weiling Liu, Duogang Ran, Jiancai Wang, and Xiaoxiao Li "Computer analysis method for determining protein relative molecular weight in gel electrophoresis images", Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); https://doi.org/10.1117/12.482926
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 5 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Proteins
Image analysis
Image processing
Digital cameras
Particles
Computing systems
Ultraviolet radiation
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top