Alternative method for simulating IPSF in two-photon laser scanning microscopy

YongJun Zhou; DeQiang Chen; Wenhao Huang; Andong Xia

doi:10.1117/12.464324

20 September 2002 Alternative Method for Simulating IPSF in Two-Photon Laser Scanning Microscopy

YongJun Zhou, DeQiang Chen, Wenhao Huang, Andong Xia

Proceedings Volume 4927, Optical Design and Testing; (2002) https://doi.org/10.1117/12.464324
Event: Photonics Asia, 2002, Shanghai, China

Abstract

The refractive-index mismatch induced by coverslips will lead to the distortion of two-photon laser scanning microscopy (TPLSM) images. Such distortion will result in the elongation of the intensity point spread function (IPSF). In order to predict the trend and extent of the distortion, we develop a new data proceeding method which combines Torok’s theory with the experimental value. This method considers the fluorescence quantum efficiency of the sample together with the detection efficiency of the system. By calculating IPSF and using our method to proceed the data, the simulation results are quite consistent with experimental results.

Citation Download Citation

YongJun Zhou, DeQiang Chen, Wenhao Huang, and Andong Xia "Alternative Method for Simulating IPSF in Two-Photon Laser Scanning Microscopy", Proc. SPIE 4927, Optical Design and Testing, (20 September 2002); https://doi.org/10.1117/12.464324

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